Detection of Oropouche virus segment S in patients and inCulex quinquefasciatus in the state of Mato Grosso, Brazil
نویسندگان
چکیده
This study aimed to investigate the circulation of Orthobunyavirus species in the state of Mato Grosso (MT) Brazil. During a dengue outbreak in 2011/2012, 529 serum samples were collected from patients with acute febrile illness with symptoms for up to five days and 387 pools of female Culex quinquefasciatus captured in 2013 were subjected to nested-reverse transcription-polymerase chain reaction for segment S of the Simbu serogroup followed by nucleotide sequencing and virus isolation in Vero cells. Patients (5/529; 0.9%) from Cuiabá (n = 3), Várzea Grande (n = 1) and Nova Mutum (n = 1) municipalities were positive for the S segment of Oropouche virus (OROV). Additionally, eight/387 Cx. quinquefasciatus pools were positive for the segment, with a minimum infection rate of 2.3. Phylogenetic analysis indicated that all the samples belong to the subgenotype Ia, presenting high homology with OROV strains obtained from humans and animals in the Brazilian Amazon. The present paper reports the first detection of an Orthobunyavirus, possibly OROV, in patients and in Cx. quinquefasciatus mosquitoes in MT. This finding reinforces the notion that arboviruses frequently reported in the Amazon Region circulate sporadically in MT during dengue outbreaks.
منابع مشابه
Chronopharmacological effects of growth hormone on the executive function and oxidative stress response in rats
Objective(s): to investigate the chronopharmacological effects of growth hormone on executive function and the oxidative stress response in rats. Materials and Methods: Fifty male Wistar rats (36-40 weeks old) had ad libitum access to water and food and were separated into four groups: diurnal control, nocturnal control, diurnal GH-treated, and nocturnal GH-treated animals. Levels of Cu, Zn sup...
متن کاملThe Mathematical Description for CoO(OH)-Assisted Hydroxylamine Electrochemical Determination in Neutral Media
The possibility of hydroxylamine electrochemical determination over a CoO(OH)-modified electrode, accompanied by complex-formation, has been studied from the theoretical point of view. The correspondent mathematical model has been developed and analyzed by means of linear stability theory and bifurcation analysis. It was shown that CoO(OH) may be applied as an efficient electrode modifier i...
متن کامل“Defense” injuries in attacks on humans by domestic dog (Canis lupus familiaris) and jaguar (Panthera onca)
This communication describes two attacks by domestic and wild carnivores in Caceres County, localized in the Pantanal area, an extensive flooded plain in Mato Grosso State, Midwest region of Brazil. The first attack took place in an urban area and was caused by a Rottweiler dog (Canis lupus familiaris) created by the family of the victim. Another attack occurred in a rural area, caused by a jag...
متن کاملImpact of an Agroforestry System on Grazing, Ruminating, and Resting Behaviors of Dairy Cows
The present study aimed to evaluate the effects of agroforestry system on the feeding behavior of dairy cows in the Brazilian Amazon region. Nineteen cross breed dairy cows (14.0±3.30 kg/d of milk yield and 450±40.1 kg body weight) were used. The following treatments were evaluated: A) animals were maintained in opened pasture of Brachiaria brizantha during all the day; B) animals were...
متن کاملHigh Levels of Sunflower Crushed in Diets for Feedlot F Lambs Depress the Performance and Carcass Quality
This study aimed to evaluate the performance, some carcass characteristics from feedlot lambs receiving sunflower crushed (SC) in the diet on partial replacement of soybean meal. The experimental design was randomized block with four levels of SC in the diet (0, 10, 20 and 30% of dry matter (DM)). There was a linear reduction of 0.02; 0.003; 0.003 and 0.17% for each percentage unit of added SC ...
متن کامل